ENTEROVAX® is a spray and/or drinking water vaccine developed to prevent viral arthritis (tenosynovitis) which causes serious leg problems and economic loss in broiler, roaster, and breeder flocks. It is ideal for use in flocks where birds are carried to heavier weights. May be safely administered to birds by spray at day of age or by water to birds 7 days of age or older, up to 21 days before slaughter.

Modified live tissue culture virus vaccine containing S-1133 strain of Reo virus

A freeze-dried pellet.

For vaccination of healthy chickens as an aid in control and prevention of reovirus induced tenosynovitis (viral arthritis) in broilers, roaster, and breeders.

When to Vaccinate
ENTEROVAX should be administered to birds 1 day of age or older.

Initial Vaccination
Spray cabinet: 1 day of age.
Water method: 7 days to 15 weeks of age.

Preparation of the Vaccine
For Spray Cabinet Use:

  1. Do not open and mix vaccine until ready for use.
  2. Only mix enough vaccine to last one hour at a time.
  3. Remove the tear-off aluminum seal from the vaccine vial without disturbing the rubber stopper.
  4. Use 70 ml of cool, distilled water to dilute each 1,000 doses of vaccine; 175ml for 2,500 doses; 350 ml for 5,000 doses.
  5. Submerge the vial in the appropriate volume of distilled water and lift the lip of the stopper to allow water to enter the vial.
  6. Reseat the stopper and shake thoroughly to dissolve the vaccine.
  7. Pour the dissolved vaccine into the diluent container.
  8. Rinse the vial twice in the diluent to remove any residual vaccine from the vial.

For Water Use:

  1. Assemble the vaccine and equipment needed to vaccinate the entire flock at one
  2. Do not open and mix the vaccine until ready for use.
  3. Remove the tear-off aluminum seal from the vaccine vial without disturbing the rubber stopper.
  4. Use cool, clean, non-chlorinated tap water to which powdered milk has been added as directed under How to Vaccinate.
  5. Holding the vial submerged in a pail of water or under a running stream of water, lift the lip of the rubber stopper so that the water (milk added) is sucked into the vial.
  6. Reseat the stopper and shake to thoroughly dissolve the vaccine.

How to Vaccinate
Spray Cabinet Method

  1. Proper spray cabinet application of this vaccine is accomplished only through the
    use of a clean, disinfectant-free spray cabinet. The spray cabinet should be adjusted
    to deliver 7 ml per 100-bird chick box. The cabinet should be operated at a pressure
    of 35-42 lbs.
    2. Using appropriate clean, sterile, disinfectant-free tubing, hook up the final mixed
    vaccine to the spray cabinet. Make sure all air bubbles are removed from the delivery
    tubing and that the proper dosage is delivered consistently with each trigger-spray.
    3. Place a chick box containing 100 chicks in the spray cabinet and trigger the
    sprayer one time to administer one dose per chick.
    4. Spray cabinet operators should wear protective goggles during vaccination to
    avoid eye contact with Newcastle vaccine.

Drinking Water Method

  1. Eliminate disinfectants from the lines 2 to 3 days prior to vaccination.
  2. Flush lines with powdered or condensed milk the night before vaccination to tie up any remaining disinfectant. Use the equivalent of 2-1/2 heaping cups of powdered milk (six 3.2 oz. packs) per gallon of stock solution or 1 cup per 50 gallons when using a vaccination tank or direct pump system for non-chlorinated water. If drinking water is chlorinated, research has shown that amounts double those above are required to neutralize up to 5 PPM of chlorine.
  3. Withhold Water Nipples or Dry Cups: Raise water lines.
    Troughs or bell drinkers: Turn off water. Monitor the drinkers closely: as soon as you see the first dry drinkers, raise all drinkers. This is the start of the “off water” time. Withhold water for 1-1/2 to 2 hours (weather dependent). The idea is to make the birds quite thirsty, but not crazy for water. Temperature, time of day, and experience with watching bird reactions will help fine-tune the water withholding time.
    Experience teaches what will work in your area.
  4. Water Requirements: Vaccine must be consumed in 1-1/2 to 2 hours.
    RULE OF THUMB: Total Daily water consumption for the flock at a given age, divided by 4, equals the amount of vaccine the birds will drink in 1-1/2 to 2 hours.
    Example: 16-day-old broilers at 70°F may drink about 16 to 18 gallons per day per 1,000 birds. Plan on 4 to 4-1/2 oz. of stock solution for each 1,000 birds to be vaccinated.
    Use non-chlorinated water for vaccination. If only chlorinated water is available, use double the powdered milk amounts indicated below to help inactivate the chlorine.

Step 1
Mix powdered milk (2-1/2 heaping cups or six 3.2 oz. packs), commercial vaccine stabilizer, or evaporated milk (ten 12 oz. cans) per gallon of stock solution. Open the vials, dip them into the milky solution, put the stopper back in and shake to dissolve the cake. Rinse the vial 3 more times after emptying the contents into the stock solution to be certain that all of the vaccine is rinsed out. Do not just drop the open vials into the stock bucket!
Step 2
Open the far end of the water line and flush the vaccine through until the milky water is flushed through the whole system. If trough or bell drinkers are used, dump any water out of the drinkers before flushing the line.
Step 3
Lower the drinkers, and allow the proportioner to operate until all of the vaccine solution has been used.
Step 4
Slowly walk the walls until vaccination is complete, encouraging all of the birds to get up and drink. Running the feed lines will also motivate the birds to get up and move around.
Step 5
Refill the stock bucket or vaccination tank with fresh water and flush the proportioner or tank system.


  1. Vaccinate only healthy birds.
  2. Do not administer this product by injection. Studies have demonstrated that injection will produce significant mortality in day-of-age birds.
  3. Do not administer using an automatic eyedrop vaccinator (e.g. Biojector- Plus) to day-of-age birds.
  4. Do not administer this product to breeders in production. Studies have demonstrated that the vaccine virus may be shed in the eggs.
  5. Do not spill or spatter the vaccine. Use entire contents of vial when first opened.
    Burn empty bottles, caps and all unused vaccine and accessories.
  6. Wash hands thoroughly after using the vaccine.
  7. All birds within a flock should be vaccinated the same day.
  8. Do not dilute or otherwise stretch the dosage.
  9. Store at 2°-7°C (35°-45°F). Do not freeze.
  10. Do not vaccinate within 21 days before slaughter.

Keep a record of vaccine type, quantity, serial number and expiration date; the date and time of vaccination; the number, age, breed, and location of the chickens; names of operators performing the vaccination and any observed reactions.